Abstract
The antimicrobial drugs benomyl and cefotaxime were used in the in vitro culture of common raspberry along with previously used preparations. We studied the efficacy of different concentrations of these drugs against microorganisms. It was experimentally found that the use of 1% sodium hypochlorite solution for 5-7 min was the most harmless to the treated plants. Higher concentrations resulted in partial or complete death of plants. The best time for in vitro introduction of common raspberry into culture is when at least 10 buds appear on the stem, during the vegetation period of plants, when raspberry growth reaches 30-40 cm. The most effective were variants with sterilisation of explants with 1% sodium hypochlorite solution for 5-7 minutes with pretreatment with antimicrobials: benomyl 500 mg, cefotaxime 0.125 mg per 1 L for 3 hours, which provides an increase in the number of viable sterile explants up to 20%. That is, we have experimentally established that the use of antimicrobials (benomyl, cefotaxime) in the sterilisation of explants in combination with sodium hypochlorite increases plant engraftment by 10%
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References
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