Abstract
Chickpeas (Cicer arietinum L.) are an important source of plant protein, but data on the biochemical composition of varieties cultivated in the Kyrgyz Republic are limited. The aim of the study was to determine the amino acid composition of the grains of three chickpea varieties (‘Kyrgyz Local’, ‘Uzbekistan 32’ and ‘Yulduz’) grown in the republic in order to assess their nutritional value. Average samples were selected according to the ISTA methodology from the 2024 harvest. Amino acid analysis was performed by high-performance liquid chromatography (HPLC) after acid hydrolysis with pre-column derivatisation with phenylisothiocyanate. The study revealed significant varietal differences in the content of individual amino acids. The highest methionine content was recorded in the ‘Kyrgyz Local’ variety (0.85 g/100 g), followed by ‘Yulduz’ (0.80 g/100 g) and ‘Uzbekistan 32’ (0.72 g/100 g). The lysine content ranged from 0.09 g/100 g (‘Yulduz’) to 0.14 g/100 g (‘Kyrgyz Local’). The dominant amino acids in all samples were aspartic acid (2.74-3.69 g/100 g) and cystine (1.53-2.70 g/100 g). The ‘Yulduz’ variety showed the highest histidine content (1.62 g/100 g), while ‘Uzbekistan 32’ had the highest tyrosine content (0.65 g/100 g). The results confirm the status of chickpeas as a valuable source of plant protein with a favourable amino acid profile and can be used in the development of balanced diets and functional foods in the Kyrgyz Republic
Keywords
References
- Arik Kibar, E.A., & Aslan, Ö. (2024). Ultrasound-assisted extraction of chickpea proteins and their functional and technological properties. Food Technology and Biotechnology, 62(4), 488-500. doi: 10.17113/ftb.62.04.24.8502.
- Asanaliev, A.Zh., Sultanbaeva, V.A., Khegay, S.V., & Sodombekov, I.S. (2017). Chickpea productivity depending on sowing dates and patterns in the foothill zone of the Chui Valley. Advances in Modern Natural Science, 3, 46-50.
- Aschemann-Witzel, J., Gantriis, R.F., Fraga, P., & Perez-Cueto, F.J.A. (2021). Plant-based food and protein trend from a business perspective: Markets, consumers, and the challenges and opportunities in the future. Critical Reviews in Food Science and Nutrition, 61(18), 3119-3128. doi: 10.1080/10408398.2020.1793730.
- Chang, Y.W., Alli, I., Konishi, Y., & Ziomek, E. (2011). Characterization of protein fractions from chickpea (Cicer arietinum L.) and oat (Avena sativa L.) seeds using proteomic techniques. Food Research International, 44(9), 3094-3104. doi: 10.1016/j.foodres.2011.08.001.
- Convention on Biological Diversity. (1992, June). Retrieved from https://www.cbd.int/.
- Dhaliwal, S.K., Salaria P., & Kaushik, Р. (2021). Pea seed proteins: A nutritional and nutraceutical update. In J.C. Jimenez-Lopez (Ed.), Grain and seed proteins functionality (Chapter 7). London: IntechOpen. doi: 10.5772/intechopen.95323.
- FAOSTAT. (n.d.). Crops and livestock products. Retrieved from https://www.fao.org/faostat/en/#data/QCL.
- Glusac, J., Isaschar-Ovdat, S., & Fishman, A. (2020). Transglutaminase modifies the physical stability and digestibility of chickpea protein-stabilized oil-in-water emulsions. Food Chemistry, 315, article number 126301. doi: 10.1016/j.foodchem.2020.126301.
- GOST 31640-2012. (2020). Feed. Methods for determining dry matter content. Retrieved from https://meganorm.ru/Data/523/52337.pdf.
- Grewal, S.K., et al. (2023) Characterization of chickpea cultivars and trait specific germplasm for grain protein content and amino acids composition and identification of potential donors for genetic improvement of its nutritional quality. Plant Genetic Resources, 20(6), 383-393. doi: 10.1017/S147926212300028X.
- International Seed Testing Association (ISTA). (n.d.). International rules for seed testing. Retrieved from https://www.seedtest.org/en/publications/international-rules-seed-testing.htmL.
- Iqbal, A., Ateeq, N., Khalil, I.A., Perveen, S., & Saleemullah, S. (2006). Physicochemical characteristics and amino acid profile of chickpea cultivars grown in Pakistan. Journal of Foodservice, 17, 94-101. doi: 10.1111/j.1745-4506.2006.00024.x.
- Köse, N., Karatas, F., & Saydam, S. (2024). Comparison of total protein and amino acid content of some legumes types. MW Journal of Science, 1(3), 8-17. doi: 10.5281/zenodo.14582470.
- Koul, B., Sharma, K., Sehgal, V., Yadav, D., Mishra, M., & Bharadwaj, C. (2022). Chickpea (Cicer arietinum L.) biology and biotechnology: From domestication to biofortification and biopharming. Plants, 11(21), article number 2926. doi: 10.3390/plants11212926.
- Liu, K., Bandara, M., Hamel, C., Knight, J.D., & Gan, Y. (2020). Intensifying crop rotations with pulse crops enhances system productivity and soil organic carbon in semi-arid environments. Field Crops Research, 248, article number 107657. doi: 10.1016/j.fcr.2019.107657.
- Ministry of Water Resources, Agriculture and Processing Industry of the Kyrgyz Republic (n.d.). Retrieved from https://agro.gov.kg/.
- National Statistical Committee of the Kyrgyz Republic (NSCKR). (n.d.). Retrieved from https://stat.gov.kg/en/.
- Olagunju, A.I., Omoba, O.S., Enujiugha, V.N., Alashi, A.M., & Aluko, R.E. (2018). Antioxidant properties, ACE/renin inhibitory activities of pigeon pea hydrolysates and effects on systolic blood pressure of spontaneously hypertensive rats. Food Science & Nutrition, 6(7), 1879-1889. doi: 10.1002/fsn3.740.
- Onder, S., Karaca, A.C., Ozcelik, B., Alamri, A.S., Ibrahim, S.A., & Galanakis, C.M. (2023). Exploring the amino-acid composition, secondary structure, and physicochemical and functional properties of chickpea protein isolates. ACS Omega, 8(1), 1486-1495. doi: 10.1021/acsomega.2c06912.
- Pankina, I.A., & Borisova, L.M. (2016). Grain legumes’ swelling and solubility of their dry solids. Scientific Journal of ITMO University. Processes and Food Production Equipment, 2, 13-20. doi: 10.17586/2310-1164-2016-9-2-13-20.
- Patil, N.D., Bains, A., Sridhar, K., Bhaswant, M., Kaur, S., Tripathi, M., Lanterbecq, D., Chawla, P., & Sharma, M. (2024). Extraction, modification, biofunctionality, and food applications of chickpea (Cicer arietinum) protein: An up-to-date review. Foods, 13(9), article number 1398. doi: 10.3390/foods13091398.
- Roy, F., Boye, J.I., & Simpson, B.K. (2010). Bioactive proteins and peptides in pulse crops: Pea, chickpea and lentil. Food Research International, 43(2), 432-442. doi: 10.1016/j.foodres.2009.09.002.
- Salaria, S., Boatwright, J.L., Johnson, N., Madurapperumage, A., Joshi, P., Thavarajah, P., Vandemark, G., & Thavarajah, D. (2023). Fatty acid composition and genome-wide associations of a chickpea (Cicer arietinum L.) diversity panel for biofortification efforts. Scientific Reports, 13, article number 14002. doi: 10.1038/s41598-023-41274-3.
- Torutaeva, E., Asanaliev, A., Prieto-Linde, M.L., Zborowska, A., Ortiz, R., Bryngelsson, T., & Garkava-Gustavsson, L. (2014). Evaluation of microsatellite-based genetic diversity, protein and mineral content in chickpea accessions grown in Kyrgyzstan. Hereditas, 151, 81-90. doi: 10.1111/hrd2.00042.
- Wang, N., & Daun, J.K. (2004). The chemical composition and nutritive value of Canadian pulses. Canadian Grain Commission Report, 28, 19-29.
- Xu, W., McClements, D.J., Xu, Z., Meng, M., Zou, Y., Chen, G., Jin, Z., & Chen, L. (2024). Optimization of emulsion properties of chickpea protein and its application in food. Journal of the American Oil Chemists’ Society, 101(10), 971-980. doi: 10.1002/aocs.12816.
- Yeasmen, N., & Orsat, V. (2025). Industrial processing of chickpeas (Cicer arietinum) for protein production. Crop Science, 65, article number e21361. doi: 10.1002/csc2.21361.
- Zia-Ul-Haq, M., Iqbal, S., Ahmad, S., Imran, M., Niaz, A., & Bhanger, M.I. (2007). Nutritional and compositional study of desi chickpea (Cicer arietinum L.) cultivars grown in Punjab, Pakistan. Food Chemistry, 105(4), 1357-1363. doi: 10.1016/j.foodchem.2007.05.004.
- Zotikov, V.I., Naumkina, T.S., Sidorenko, V.S., Gryadunova, N.V., & Naumkin, V.V. (2016). Leguminous crops – an important factor in sustainable, environmentally-oriented agriculture. Legumes and Groat Crops, 17(1), 6-13.